Blood Specimen Collection Venipuncture Procedure

Required Items

1. Blood collection tube
2. Barrel Syringe
3. Hypodermic needle with safety cap
4. Blood Transfer device
5. gauze
6. Alcohol Swabs
7. Non Allergic Plaster
8. Tourniquet

Position the patient in a chair, or sitting or lying on a bed.

Wash your hands.

Select a suitable site for venipuncture, by placing the tourniquet 3 to 4 inches above the selected puncture site on the patient.

Do not put the tourniquet on too tightly or leave it on the patient longer than 1 minute.

Next, put on non-latex gloves, and palpate for a vein.

When a vein is selected, cleanse the area in a circular motion, beginning at the site and working outward. Allow the area to air dry. After the area is cleansed, it should not be touched or palpated again. If you find it necessary to reevaluate the site by palpation, the area needs to be re-cleansed before the venipuncture is performed.

Ask the patient to make a fist; avoid “pumping the fist.” Grasp the patient’s arm firmly using your thumb to draw the skin taut and anchor the vein. Swiftly insert the needle through the skin into the lumen of the vein. The needle should form a 15-30 degree angle with the arm surface. Avoid excess probing.

When the tube is filling, remove the tourniquet.

Remove the needle from the patient's arm using a swift backward motion.

Place gauze immediately on the puncture site. Apply and hold adequate pressure to avoid formation of a hematoma. After holding pressure for 1-2 minutes, tape a fresh piece of gauze or Band-Aid to the puncture site.


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